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Fig. 5 | Col6a1cre and Twist2cre fibroblast-specific targeting in the ileum. a H&E images of 4-µm-thick ileal paraffin sections and immunofluorescent confocal ima- ges from 12-µm-thick ileal cryosections, stained with DAPI and antibodies against Podoplanin (PDPN) and <t>CD45.</t> Images are representative of 3 mice per condtition. b, c Flow cytometry analysis of Live/Lin−/Pdpn+ ileal fibroblasts from the ileum of 2–3-month-old Twist2cre R26mT/mG (n = 7 mice/population) (b) and Col6a1cre R26mT/mG
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Fig. 5 | Col6a1cre and Twist2cre fibroblast-specific targeting in the ileum. a H&E images of 4-µm-thick ileal paraffin sections and immunofluorescent confocal ima- ges from 12-µm-thick ileal cryosections, stained with DAPI and antibodies against Podoplanin (PDPN) and CD45. Images are representative of 3 mice per condtition. b, c Flow cytometry analysis of Live/Lin−/Pdpn+ ileal fibroblasts from the ileum of 2–3-month-old Twist2cre R26mT/mG (n = 7 mice/population) (b) and Col6a1cre R26mT/mG

Journal: Nature communications

Article Title: Different fibroblast subtypes propel spatially defined ileal inflammation through TNFR1 signalling in murine ileitis.

doi: 10.1038/s41467-025-57570-7

Figure Lengend Snippet: Fig. 5 | Col6a1cre and Twist2cre fibroblast-specific targeting in the ileum. a H&E images of 4-µm-thick ileal paraffin sections and immunofluorescent confocal ima- ges from 12-µm-thick ileal cryosections, stained with DAPI and antibodies against Podoplanin (PDPN) and CD45. Images are representative of 3 mice per condtition. b, c Flow cytometry analysis of Live/Lin−/Pdpn+ ileal fibroblasts from the ileum of 2–3-month-old Twist2cre R26mT/mG (n = 7 mice/population) (b) and Col6a1cre R26mT/mG

Article Snippet: Tissue permeabilization was achieved with 0.03% Triton X-100 (Acros Organics, 2156825000) in PBS for 10minutes, followed by blocking in PBS containing 1% albumin for 1 h. Immunofluorescence staining on these sections involved primary antibodies against anti-CD45 (AF114, R&D Systems), Biotin-conjugated antipodoplanin (127403, Biolegend), anti-CD68 (PAS-78996, ThermoFisher), and anti-collagen IV (ab6586, Abcam).

Techniques: Staining, Flow Cytometry